Black Int
Selection of Marine Azotobacter as Biofertilizer for Soil Applicatioin
The use of chemical fertilizer is the cause of soil deterioration and water pollution. Biofertilizer, most of which are nitrogen-fixing microorganisms are considered to be suitable alternative source of plant nutrition Azotobacter fixes atmospheric nitrogen in the soil and makes it available to the plants. The objective of the study was to check the stability of marine azotobacter in the soil. In this study totally 100 samples were collected from the region of Palk Bay (thondi) at the death of 1 – 2m at the interval of 20days. At each time 10 – 15 marine and sediment samples were collected. Out of 70 marine water sample, all the samples showed the presence of Azotobacter, but only 23 marine sediment showed the presence of Azotobacter when grown on selective medium. Pot culture experiments were carried out, to determine the N2 fixing ability of marine Azotobacter in garden soil by assessing the growth of black gram. Five efficient strains were selected based on acetylene reduction assay for the experiment. Healthy black gram seeds were mixed with Azotobacter inoculum containing 1 × 109 cells seeds were immediately sown int the parts. Seed germination was calculated after 4 days of sowing which showed 85% germination in each pot than the uninoculated control. Shoot and root length of the plants were measured, which ranged from 21.4 – 26.3cm and 7.6 – 12.2cm respectively. The chlorophyll content was 0.41294-0.8587OD at 645 and 663nm. This study reveals that black gram inoculated with Azotobacter strain showed significant increase in shoot and root length and chlorophyll content as compared with non-inoculated contro
About the Author
RAJESWARI K,RUPESH.H, KARTHICK.A AND KALAIGANDHI.V
DR.GRD CS
COIMBATORE
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